[B]the reason this article below is so interesting is because it shows that by possibly affecting/ increasing mitochondrial biogenesis, ovarian function can be restored to some degree in age related decline in fertility. Perhaps by using hydroxytyrosol rich olive leaf tea 3 months before trying to conceive can assist. Hydroxytyrosol increases mitochondria in cells.
BACKGROUND: Mitochondrial biogenesis and bioenergetics play an important role in oocyte maturation and embryo development. We have investigated the relationship between defective mitochondrial biogenesis and the lack of oocyte maturity observed during IVF procedures with patients suffering from ovarian dystrophy and ovarian insufficiency. METHODS: We used real-time quantitative PCR to quantify mitochondrial DNA (mtDNA) in 116 oocytes obtained from 47 women undergoing the ICSI procedure. We compared the mtDNA content of oocytes from women with a normal ovarian profile with that of oocytes from women with ovarian dystrophy and ovarian insufficiency. RESULTS: We found an average of 256 000±213 000 mitochondrial genomes per cell. The mean mtDNA copy number was not significantly different in ovarian dystrophy compared with controls, but it was significantly lower in oocytes from women with ovarian insufficiency (100 000±99 000, P<0.0001). CONCLUSIONS: Our results suggest that low mtDNA content is associated with the impaired oocyte quality observed in ovarian insufficiency.
IVF mitochondrial DNA oocyte quality ovarian insufficiency
Although assisted reproductive technology has greatly progressed over the past two decades, the average rate of pregnancy obtained with IVF by embryo transfer is still low at 25% (FIVNAT,