PGS/PGD on Frozen Blasts?


#1

Hi Ladies,

Sending everyone sticky vibes.

Anyone know anyone who’se done PGS or PGD on frozen blasts? What was the outcome? Thoughts on it?

Specific situation would include:

  1. thawing frozen blasts to take cells for testing
  2. re-freezing blasts immediately
  3. thawing blasts again for transfer after getting results

TIA!


#2

Hi,

I had my 9 embryos tested on Day 5 and then frozen. 2 of them did not have enough DNA to test and had to be rethawed, rebiopsed and then refrozen.

I would go for it. Both of my embies made the thaw, both came back normal and were refrozen. I did lots of research and found numerous articles that as long as they were healthy normal embryos they were not affected by refreezing process. Vitrification freezing process only.

I am glad we did this as 5 of the 9 were chromsomally abnormal. We would have only had 2 embryos then.

I am transferring one of them in 2 weeks. So the jury is out but I am hoping they do ok.

Best of luck!

Rach


#3

Thanks for sharing your experience Rach. I’ll be following your progress. I think we’ve decided to do it.

I wish you much success with your transfer and loads of :bsv:


#4

We had 7 frozen embryos that we decided to biopsy. 2 didn’t survive the thaw so we had the other 5 tested. 1 came back normal, 2 abnormal and 2 not enough DNA.

For transfer we thawed the normal one and the 2 with not enough DNA - the normal one thawed just fine along with 1 of the other ones and the other one didn’t survive the thaw the 2nd time.


#5

falltime, thanks so much for coming on to share your experience. I appreciate it. :grouphug:


#6

Just did my FET today! We transferred 1 embryo. Everything looked good and the thaw went well!! I wanted to give a shout out to CCRM’s lab. The embryo that we transfered today was frozen back in April 2012. At the time we didn’t do CCS testing. Then I got pregnant with twins and miscarried. We did a D&C and realized it was chromosome issues. So we decided to thaw my remaining frozen emboys and CCS tested them-then had to refreeze them. Today thawed 1 and Transfered!. I was scared with the whole process! My lab is amazing. It’s still early so I don’t know if it worked but I do know they took great care of my emborys.

Good luck with whatever you decide!!!


#7

JenE, praying all goes smoothly with your transfer. :pray: Thanks for sharing your experience. Sounds like CCRM is an amazing place. Happy for you that they took great care of your embabies.


#8

Hi everyone, Just wanted to share my experience so far with doing PGS on my frozen blasts. We did the test on my 7 remaining embryos. all the embryos thawed, got tested (cell extracted), and refroze without any issue. Only 2 were normal. I transferred one normal one on 9/12. It thawed just fine the second time around and the embryologist said it was soon very well. On 9/23, today, I got my first beta and its positive at a 378 level. Hope this helps someone sometime. Hugs to all and much success.


#9

First, congratulations!!! Second, thanks for sharing your experience. I am doing Lupron now and will hopefully do FET in Oct. Question: how long between thaw-test-thaw? How long did it take for you to get test results?


#10

Thanks! I’m not exactly sure which day the lab thawed the embryos for the PGS test, but on 8/6, the genetics lab sent me a note saying they had received my patient intake form from my clinic. I got the test results by around 8/12 I believe. Then my transfer was on 9/12. Wishing you success with your FET in October.


#11

Thanks for sharing this…I wish I had seen this before I did my transfer on 9/24. I struggled with whether we should have done the PGS on the only 3 frozen embies… Does this mean the results that this is 100% accurate…
So happy for you…we have 1 frozen left but we have 2 that were implanted so we are hoping for positive results.


#12

Bbrfh28, all the forms have a caveat saying that there is always a chance for error. Typical stuff. Wishing you luck with the 2 you transferred.